Immobilization of glucoamylase on polyamide nets

✍ Scribed by E. Miller

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 542 KB
• Volume: 18
• Category: Article
• ISSN: 0138-4988
• DOI: 10.1002/abio.370180208

✦ Synopsis

The formation of reactive groups on polyamide nets (nylon 6) and the subsequent immobilization of glucoamylase were investigated. Different mesh sizes of the nets and two chemical methods of enzyme couplingi) partial hydrolysis of the polyamide with subsequent glutaraldehyde binding and ii) 0-alkylation of the carrier using a treatment with a benzene-methyl sulphate mixturewere used

The reactivity of immobilized glucoamylase (GA) was tested by hydrolysis reactions using 1% starch solutions. The highest reactivity (140 pg glc/(min x cm2) was obtained for methylated nylon samples attached to a glass rod and by coupling glucoamylase on the nylon surface which had been treated with lysine and glutaraldehyde. This method resulted in a more reactive and more stable preparation of immobilized glucoamylase as compared to a simpler method of coupling glutaraldehyde to partially hydrolyzed nylon.