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Immobilization of Candida rugosa lipase on colloidal gas aphrons (CGAs)

✍ Scribed by P. J. O'Connell; J. Varley

Publisher: John Wiley and Sons
Year: 2001
Tongue: English
Weight: 121 KB
Volume: 74
Category: Article
ISSN: 0006-3592
DOI: 10.1002/bit.1116

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✦ Synopsis

Abstract

A novel technique for immobilization of Candida rugosa lipase onto anionic colloidal gas aphrons (CGAs) is described. CGAs are spherical microbubbles (10–100 μm) composed of an inner gas core surrounded by a surfactant shell. In this initial study, greater than 80% lipase (w/w) was effectively retained on the CGAs. Leakage of protein from the CGAs and the activity of the adsorbed lipase decreased with increasing enzyme loading; this indicates that multilayers of lipase may be adsorbing onto the CGAs. The CGA‐immobilised lipase displayed normal Michaelis‐Menten dependence on substrate concentration and also exhibited greater activity than the free enzyme. © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 74: 264–269, 2001.

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