Immobilization of Candida antarctica lipase B by adsorption in organic medium

✍ Scribed by Jiangna Sun; Yanjun Jiang; Liya Zhou; Jing Gao

• Book ID: 104064756
• Publisher: Elsevier
• Year: 2010
• Tongue: English
• Weight: 245 KB
• Volume: 27
• Category: Article
• ISSN: 1871-6784
• DOI: 10.1016/j.nbt.2009.12.001

✦ Synopsis

Candida antarctica lipase B (CALB) was immobilized on the macroporous resin by physical adsorption in organic medium. The immobilization was performed in 5 mL isooctane, and the immobilization conditions were optimized. The results were achieved with the mass ratio of lipase to support 1:80, the buffer of pH 6.0, initial addition of PBS 75 microL, and immobilization time of two hours at 30 degrees C. Under the optimal conditions, the activity recovery was 83.3%. IM-CALB presented enhanced pH and thermal stability compared to the free lipase, and showed comparable stability with the commercial Novozym 435, after 7 times repeated use for catalyzing the synthesis of ethyl lactate, 56.9% of its initial activity was retained, and only 24.7% was retained when used for catalyzing the hydrolysis of olive oil.