Immobilisation of biomolecules for biosensors

✍ Scribed by Barlen, Benjamin ;Mazumdar, Saikat Datta ;Keusgen, Michael

• Book ID: 105365197
• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 599 KB
• Volume: 206
• Category: Article
• ISSN: 0031-8965
• DOI: 10.1002/pssa.200880478

✦ Synopsis

Abstract

Immobilisation of proteins, mainly enzymes and antibodies, is one of the most crucial steps in the development of biosensors. Unspecific methods as well as site‐directed strategies have been developed in order to obtain highest functionality and stability. Different methods for immobilisation of antibodies were tested to develop a surface plasmon resonance (SPR) biosensor for the detection of Salmonella. It turned out, that best results in terms of capturing and detection of Salmonella were achieved by immobilisation via hydrophobic interaction, using a sensor surface coated with C~18~‐alkyl chains allowing integration of hydrophobic parts of the antibody. (© 2009 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)