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Imaging theory of nonlinear second harmonic and third harmonic generations in confocal microscopy

✍ Scribed by Zhilie Tang; Da Xing; Songhao Liu

Book ID
111784702
Publisher
Science in China Press (SCP)
Year
2004
Tongue
English
Weight
518 KB
Volume
47
Category
Article
ISSN
1672-1799

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Simultaneous two-photon excited Β―uorescence (TPF) and second-harmonic generation (SHG) imaging is demonstrated using a single femtosecond laser and a scanning microscope. This composite nonlinear microscopic technique was applied to imaging DNA and chromosomes, and it was shown that the two differen