Using a setup based on fluorescence microscopy we study fluorescence spectra of single chromophores. Being point like emission sources single molecules can be focused to the entrance slit of a spectrometer. Even with narrow slit width the light passes the system with almost no loss. We show that, ma
Imaging Single Molecules in Three Dimensions
✍ Scribed by Gerhard J. Schütz; Markus Axmann; Hansgeorg Schindler
- Publisher
- John Wiley and Sons
- Year
- 2001
- Tongue
- English
- Weight
- 488 KB
- Volume
- 2
- Category
- Article
- ISSN
- 1438-5163
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
We report on far-field fluorescence imaging of single molecules in spherical polymer microparticles produced from solution by using microdroplet techniques. The fluorescence photobleaching quantum yields of rhodamine 6G in a common water-soluble polymer (polyvinyl alcohol) are at least five times sm
In order to image single molecule fluorescence (SMF), an optimized optical microscope station is required as well as a high sensitivity detection system. This talk describes the current trends in detectors for SMF using back illuminated CCD technology as well as image intensified technology. A compa