Imaging proteins inside cells with fluorescent tags
β Scribed by Georgeta Crivat; Justin W. Taraska
- Book ID
- 113932411
- Publisher
- Elsevier Science
- Year
- 2012
- Tongue
- English
- Weight
- 657 KB
- Volume
- 30
- Category
- Article
- ISSN
- 0167-7799
No coin nor oath required. For personal study only.
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## Abstract Technologies to visualize cellular structures and dynamics enable cell biologists to gain insight into complex biological processes. Currently, fluorescent proteins are used routinely to investigate the behavior of proteins in live cells. Chemical biology techniques for selective labeli
A cell's biochemistry is now known to be the biochemistry of molecular machines, that is, protein complexes that are assembled and dismantled in particular locations within the cell as needed. One important element in our understanding has been the ability to begin to see where proteins are in cells
A vast colour palette of monomeric fluorescent proteins has been developed to investigate protein localization, motility and interactions. However, low brightness has remained a problem in far-red variants, which hampers multicolour labelling and whole-body imaging techniques. In the present paper,