โ Scribed by Yumiko Yoshiki; Tetsuo Iida; Yoshinobu Akiyama; Kazuyoshi Okubo; Hitoshi Matsumoto; Michikatsu Sato
No coin nor oath required. For personal study only.
An imaging system for the hydroperoxide and hydrogen donor was developed by photon emission from the reactive oxygen species-hydrogen donor-KHCO 3 -MeCHO system. Photon intensity (luminance, cd/m 2 ) showed a linear correlation with the concentration of some hydroperoxides [hydrogen peroxide (H 2 O 2 ), tert-butyl hydroperoxide and methyl ethyl ketone peroxide] and that of hydrogen donors (catechins and anthocyanins). The linear relationship between photon intensity and concentration was observed in polyphenol-rich samples, such as teas, berries and currants. Photon intensity from the H 2 O 2 /polyphenol-rich sample-KHCO 3 -MeCHO system corresponded with H 2 O 2 -scavenging activity, rather than with polyphenol contents. It is possible to classify hydrogen donor species according to absorption features using spectral analysis (gallic acid, E max 645 nm, catechins, E max 610 nm; anthocyanins, E max 690-700 nm). This method is a simple and sensitive detection system for hydroperoxide-and H 2 O 2 -scavenging substances.
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