## Abstract A new algorithm is presented for the detection of single, fluorescence‐labeled proteins in the analysis of images from living cells. It is especially suited for images with just a few (<1 per 10 μm^2^) fluorescence peaks from individual proteins with high background and noise (signal to
✦ LIBER ✦
Imaging of Conformational Changes of Proteins with a New Environment-Sensitive Fluorescent Probe Designed for Site-Specific Labeling of Recombinant Proteins in Live Cells
✍ Scribed by Nakanishi, Jun; Nakajima, Takahiro; Sato, Moritoshi; Ozawa, Takeaki; Tohda, Kohji; Umezawa, Yoshio
- Book ID
- 120620734
- Publisher
- American Chemical Society
- Year
- 2001
- Tongue
- English
- Weight
- 186 KB
- Volume
- 73
- Category
- Article
- ISSN
- 0003-2700
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## Abstract **Designer label**: A newly developed polarity‐sensitive fluorescent probe (DBHA) was combined with a tyrosine‐specific labelling method that uses transition metal catalysis, and was successfully used in local structural analysis of the Tyr108 domain in Cu/Zn superoxide dismutase (SOD;