Imaging Membrane Protein Helical Wheels

✍ Scribed by J. Wang; J. Denny; C. Tian; S. Kim; Y. Mo; F. Kovacs; Z. Song; K. Nishimura; Z. Gan; R. Fu; J.R. Quine; T.A. Cross

• Book ID: 102601287
• Publisher: Elsevier Science
• Year: 2000
• Tongue: English
• Weight: 109 KB
• Volume: 144
• Category: Article
• ISSN: 1090-7807
• DOI: 10.1006/jmre.2000.2037

✦ Synopsis

Resonance patterns have been observed in 2D solid-state NMR spectra of the transmembrane segment of M2 protein from Influenza A virus in oriented samples reflecting the helical wheel of this ␣-helix. The center of this pattern uniquely defines the helical tilt with respect to the bilayer normal without a need for resonance assignments. The distribution of resonances from amino acid specific labels around the "PISA wheel" defines the rotational orientation of the helix and yields preliminary site-specific assignments. With assignments high-resolution structural detail, such as differences in tilt and rotational orientation along the helical axis leading to an assessment of helical coiling, can be obtained.