Imaging Mass Spectrometry: Protocols for Mass Microscopy

✍ Scribed by Mitsutoshi Setou (auth.), Mitsutoshi Setou (eds.)

Publisher: Springer Tokyo
Year: 2010
Tongue: English
Leaves: 252
Series: SpringerLink: Springer e-Books
Edition: 1
Category: Library

No coin nor oath required. For personal study only.

✦ Synopsis

Addressing the widespread need for a practical guide to imaging mass spectrometry (IMS), this book presents the protocols of IMS technology. As that technology expands, research groups around the world continue its development. Pharmaceutical companies are using IMS for drug analyses to study pharmacokinetics and medical properties of drugs. Drug research and disease-related biomarker screening are experiencing greater use of this technology, with a concurrent increase in the number of researchers in academia and industry interested in wider applications of IMS. Intended for beginners or those with limited experience with IMS technology, this book provides practical details and instructions needed for immediate know-how, including the preparation of animal tissue samples, the application of a matrix, instrumental operations, and data analysis, among others. By describing the foundations of IMS, this volume contributes to the ongoing development of the field and to progress in human health.

✦ Table of Contents

Front Matter....Pages i-x
Front Matter....Pages 1-1
IMS as an Historical Innovation....Pages 3-7
Front Matter....Pages 9-9
Guide to Planning the Sample Preparation Step....Pages 11-30
Front Matter....Pages 31-31
Animal Care and Tissue Sample Extraction for IMS....Pages 33-39
Preparing Biological Tissue Sections for Imaging Mass Spectrometry....Pages 41-54
Matrix Choice....Pages 55-69
Methods of Matrix Application....Pages 71-85
Matrix Application with ImagePrep....Pages 87-91
Front Matter....Pages 93-93
Method of Operating AXIMA-QIT as Imaging Instrument....Pages 95-109
Front Matter....Pages 111-111
Ion Image Reconstruction Using BioMap Software....Pages 113-126
Statistical Procedure for IMS Data Analysis....Pages 127-142
Statistical Analysis of IMS Dataset with ClinproTool Software....Pages 143-155
Front Matter....Pages 157-157
Imaging of Cultured Cells by Mass Spectrometry....Pages 159-168
TLC-Blot-MALDI-IMS....Pages 169-177
Front Matter....Pages 179-179
Applied Biosystems....Pages 181-198
Bruker Daltonics....Pages 199-208
Imaging and Molecular Identification of Biomolecules on Tissue Sections with AXIMA-QIT: Shimadzu Corporation....Pages 209-219
MALDI Imaging with Ion-Mobility MS: Waters Corporation....Pages 221-231
Front Matter....Pages 233-233
Comparison of SIMS and MALDI for Mass Spectrometric Imaging....Pages 235-257
Back Matter....Pages 259-262

✦ Subjects

Biological Microscopy; Cell Biology; Biological Techniques; Imaging / Radiology; Mass Spectrometry; Pharmacology/Toxicology

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