Imaging mass spectrometry: a new tool to investigate the spatial organization of peptides and proteins in mammalian tissue sections

✍ Scribed by Pierre Chaurand; Sarah A Schwartz; Richard M Caprioli

• Book ID: 104414710
• Publisher: Elsevier Science
• Year: 2002
• Tongue: English
• Weight: 454 KB
• Volume: 6
• Category: Article
• ISSN: 1367-5931
• DOI: 10.1016/s1367-5931(02)00370-8

✦ Synopsis

MALDI MS imaging mass spectrometry can be used to map the distribution of targeted compounds in tissue sections with a spatial resolution currently of about 50 microm, providing important molecular information in many areas of biological research. After matrix application, a raster of a section by the laser beam yields ions from compounds in a tissue mass-to-charge range from 1000 to over 100000. Two-dimensional intensity maps can then be reconstructed to provide specific molecular images of a tissue.