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Imaging living mice using a 1-T compact MRI system

✍ Scribed by Yusuke Inoue; Yukihiro Nomura; Tomoyuki Haishi; Kohki Yoshikawa; Takahiro Seki; Kyoko Tsukiyama-Kohara; Chieko Kai; Toshiyuki Okubo; Kuni Ohtomo

Publisher
John Wiley and Sons
Year
2006
Tongue
English
Weight
801 KB
Volume
24
Category
Article
ISSN
1053-1807

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✦ Synopsis

Abstract

Purpose

To determine the feasibility of imaging living mice with a 1‐T compact MRI system and investigate appropriate imaging techniques for use in routine animal experiments.

Materials and Methods

An MRI system consisting of a 1‐T permanent magnet and compact console was used. Images of the entire trunks of living mice were obtained on the system using a T1‐weighted three‐dimensional fast low‐angle shot (3D FLASH) sequence, and image quality was evaluated in relation to imaging techniques.

Results

Restraint of respiratory motion improved the image quality. Decreasing the slice thickness reduced artificial inhomogeneity in signal intensity (SI). Substantial effects of TR and FA on image quality were also demonstrated. With the determined techniques, images covering the entire trunk with a voxel size of 0.26 × 0.26 × 0.52 mm were acquired in an acquisition time of five minutes 28 seconds and a total experiment time of <20 minutes, and various organs and subcutaneous tumors were clearly visualized.

Conclusion

The compact MRI system provides images of living mice with acceptable quality in a reasonable time. Considering its convenience, it appears to be suitable for use in routine mouse experiments. J. Magn. Reson. Imaging 2006. © 2006 Wiley‐Liss, Inc.

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