Imaging H217O distribution in a phantom and measurement of metabolically produced H217O in live mice by proton NMR
✍ Scribed by Itamar Ronen; Jing-Huei Lee; Hellmut Merkle; Kamil Ugurbil; Gil Navon
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 327 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0952-3480
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✦ Synopsis
Previously we suggested a method for indirect detection of H 2 17 O via a combination of 1 H spin-echo sequence and 17 O decoupling. The method is based on the shortening of the water proton T 2 due to the residual spin-spin coupling with the 17 O nucleus, and on our finding that this shortening is abolished by applying an radio frequency field at the 17 O resonance frequency. In the present work we demonstrate how the method can be used for imaging low concentrations of H 2 17 O, and report for the first time an application of this method to in vivo detection of H 2 17 O in live newborn mice, where the H 2 17 O was introduced either directly i.p. or by inhalation of 17 O 2 where it was formed by oxidative metabolic activity.