Imaging Gene Expression: Methods and Protocols

✍ Scribed by Yaron Shav-Tal

• Publisher: Springer New York;Humana
• Year: 2019
• Tongue: English
• Leaves: 273
• Series: Methods in Molecular Biology 2038
• Edition: 2nd ed. 2019
• Category: Library

✦ Synopsis

This volume explores the latest updates on microscopy approaches and techniques used by scientists studying in the field of gene expression imaging. These updates cover the technical design of the experiments and the expected outcomes. The chapters in this book are divided into two parts: Part One looks at the output of a gene, in particular the RNA molecules that are copied from the gene itself; and Part Two focuses on chromosomes, chromatin, and factors that bind DNA. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Imaging Gene Expression: Methods and Protocols, Second Edition is a valuable resource for any researcher interested in learning more about this evolving and important field.

✦ Table of Contents

Front Matter ....Pages i-xiii
Front Matter ....Pages 1-1
Imaging Single mRNA Molecules in Mammalian Cells Using an Optimized MS2-MCP System (Maria Vera, Evelina Tutucci, Robert H. Singer)....Pages 3-20
Live-Cell Imaging of Long Noncoding RNAs Using Molecular Beacons (Yachen Ying, Shiqi Mao, Christopher J. Krueger, Antony K. Chen)....Pages 21-33
Real-Time Observation of Localization and Expression (ROLEX) System for Live Imaging of the Transcriptional Activity and Nuclear Position of a Specific Endogenous Gene (Hiroshi Ochiai)....Pages 35-45
Visualization of Single mRNAs in Live Neurons (Jae Youn Shim, Byung Hun Lee, Hye Yoon Park)....Pages 47-61
Lighting Up Gene Activation in Living Drosophila Embryos (Carola Fernandez, Mounia Lagha)....Pages 63-74
Quantification of mRNA Turnover in Living Cells: A Pipeline for TREAT Data Analysis (Franka Voigt, Jan Eglinger, Jeffrey A. Chao)....Pages 75-88
Imaging rRNA Methylation in Bacteria by MR-FISH (Kristina A. Ganzinger, Martin R. Challand, James Spencer, David Klenerman, Rohan T. Ranasinghe)....Pages 89-107
Detection of mRNA Transfer Between Mammalian Cells in Coculture by Single-Molecule Fluorescent In Situ Hybridization (smFISH) (Gal Haimovich, Jeffrey E. Gerst)....Pages 109-129
Live-Cell Imaging of mRNP–NPC Interactions in Budding Yeast (Azra Lari, Farzin Farzam, Pierre Bensidoun, Marlene Oeffinger, Daniel Zenklusen, David Grunwald et al.)....Pages 131-150
Detection of mRNAs Anchored to the Nuclear Envelope During Export Inhibition in Living Cells (Rakefet Ben-Yishay, Yaron Shav-Tal)....Pages 151-163
Front Matter ....Pages 165-165
Programmable Chromosome Painting with Oligopaints (Son C. Nguyen, Eric F. Joyce)....Pages 167-180
Imaging Chromosome Territory and Gene Loci Positions in Cells Grown on Soft Matrices (Roopali Pradhan, Kundan Sengupta)....Pages 181-197
Targeted Manipulation/Repositioning of Subcellular Structures and Molecules (Kathrin S. Heinz, M. Cristina Cardoso)....Pages 199-208
Visualization of Endogenous Transcription Factors in Single Cells Using an Antibody Electroporation-Based Imaging Approach (Sascha Conic, Dominique Desplancq, Alexia Ferrand, Nacho Molina, Etienne Weiss, László Tora)....Pages 209-221
Monitoring Transcription Factor Oligomerization in Single Living Cells by Number and Brightness Analysis (Eugenia Cammarota, Davide Mazza)....Pages 223-237
Visualizing Transcription Factor Binding on Mitotic Chromosomes Using Single-Molecule Live-Cell Imaging (James Z. J. Kwan, Thomas F. Nguyen, Sheila S. Teves)....Pages 239-250
Light-Induced Transcription Activation for Time-Lapse Microscopy Experiments in Living Cells (Jorge Trojanowski, Anne Rademacher, Fabian Erdel, Karsten Rippe)....Pages 251-270
Back Matter ....Pages 271-272

✦ Subjects

Biomedicine; Gene Expression; Biological Microscopy