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Imaging and Tracking of Single GFP Molecules in Solution

✍ Scribed by Kubitscheck, Ulrich; Kückmann, Oliver; Kues, Thorsten; Peters, Reiner

Book ID
118513928
Publisher
Biophysical Society
Year
2000
Tongue
English
Weight
499 KB
Volume
78
Category
Article
ISSN
0006-3495

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Using a setup based on fluorescence microscopy we study fluorescence spectra of single chromophores. Being point like emission sources single molecules can be focused to the entrance slit of a spectrometer. Even with narrow slit width the light passes the system with almost no loss. We show that, ma