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Illegitimate recombination: Role of type II DNA topoisomerase

✍ Scribed by Ikeda, H


Book ID
122061201
Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
533 KB
Volume
21
Category
Article
ISSN
0065-227X

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πŸ“œ SIMILAR VOLUMES


Illegitimate recombination mediated by T
✍ Ikeda, Hideo πŸ“‚ Article πŸ“… 1986 πŸ› Springer 🌐 English βš– 332 KB

Illegitimate recombination dependent on T4 DNA topoisomerase in a cell-free system has recently been described. In that work, recombinants between two phage lambda DNA molecules were produced by the topoisomerase alone, without an Escherichia coli extract. In this paper, it is shown that recombinati

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## Abstract We have developed a plasmid test system to study recombination in vitro and in mammalian cells in vivo, and to analyze the possible role of DNA topoisomerase II. The system is based on a plasmid construct containing an inducible marker gene __ccdB__ (β€œkiller” (__KIL__) gene) whose produ