IL2 effect on T lymphocyte phenotype of PBPC from healthy donors and patients
✍ Scribed by M.J. Baptista; A.J. Almeida; S. Roncon; A. Évila; A. Campos; P. Pimentel; A. Carvalhais; I.L. Barbosa
- Book ID
- 117658211
- Publisher
- Elsevier Science
- Year
- 2001
- Tongue
- English
- Weight
- 178 KB
- Volume
- 37
- Category
- Article
- ISSN
- 0959-8049
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Anticoagulant and room temperature storage of peripheral blood was evaluated in healthy and diseased persons (AIDS and CLL) for its effect upon lymphocyte phenotyping by flow cytometry. Whole blood was stored in either Li heparin, EDTA, or ACD for up to 96 hours. Aliquots at 5 time intervals were ev
## Abstract Lymphocytes from 25 healthy donors were separated into T‐ and non‐T‐fractions by means of E and EAC rosette‐formation. The unfractionated lymphocyte population was tested simultaneously with E and non‐EAC rosette‐forming cells (T‐cells) and EAC and non‐E rosette‐forming cells (non‐T cel