IL1RN polymorphic gene and cagA-positive status independently increase the risk of noncardia gastric carcinoma
✍ Scribed by Gifone Aguiar Rocha; Juliana Becattini Guerra; Andreia Maria Camargos Rocha; Ivan Euclides Borges Saraiva; Deborah Alves da Silva; Celso Affonso de Oliveira; Dulciene Maria Magalhães Queiroz
- Publisher
- John Wiley and Sons
- Year
- 2005
- Tongue
- French
- Weight
- 99 KB
- Volume
- 115
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
Abstract
__Helicobacter pylori cag__A‐positive strains and host cytokine proinflammatory polymorphisms have been associated with gastric carcinoma. However, the individual role of each factor has not been evaluated yet. Our aim was to evaluate whether IL‐1 gene cluster and tumor necrosis factor‐α (TNFA)‐307 polymorphisms, as well as __cag__A‐positive status, are associated with gastric carcinoma in a non‐Caucasian population by analyzing the data in logistic regression models. We evaluated 166 patients with noncardia gastric carcinoma and 541 blood donors. Among them, 702 were successfully genotyped for all cytokine studied: 166 with gastric carcinoma and 536 controls. The carcinoma patients were considered to be H. pylori‐positive if culture alone or 2 among preformed urease test, stained smear or histologic section, serology, polymerase chain reaction (PCR) for __ure__A and urea breath test were positive. In blood donors, H. pylori status was based on enzyme‐linked immunosorbent assay. The __cag__A status was determined by PCR or serology. IL1B‐511/‐31, IL1RN (interleukin‐1 receptor antagonist) and TNFA‐307 polymorphisms were genotyped by PCR, PCR with restriction fragment length polymorphism, or PCR with confronting 2‐pair primers. We found that the __IL1RN__2 polymorphic allele (OR = 1.93) was associated with noncardia gastric carcinoma, even after inclusion of age, gender and __cag__A status in the logistic models. However, the __cag__A‐positive status was the strongest independent factor associated with gastric carcinoma (OR = 11.89). The other polymorphisms were not significantly associated with the disease when they were evaluated in logistic models. This study provides evidence supporting the independent associations of __cag__A‐positive H. pylori status and IL1RN polymorphisms with noncardia gastric carcinoma. © 2005 Wiley‐Liss, Inc.