IL-15 dependent allograft rejection

✍ Scribed by Ascher, N L

• Book ID: 102472683
• Publisher: Wiley (John Wiley & Sons)
• Year: 1997
• Tongue: English
• Weight: 70 KB
• Volume: 3
• Category: Article
• ISSN: 1074-3022
• DOI: 10.1002/lt.500030109

✦ Synopsis

IL-15, a novel growth factor made by a variety of cells, stimulates T-cell proliferation in a fashion similar to IL-2. IL-2 transcripts are not routinely found in rejecting human renal allografts at the time of clinically evident rejection. However, T-cell proliferation continues as the rejection progresses. We postulated that IL-15 may be actively transcribed during clinical rejection and account, at least in part, for the ongoing T-cell expansion. RNA was extracted from renal biopsies and reverse transcribed to cDNA, which was used as template for competitive PCR. IL-2 mRNA was detected in just 3 of the 45 biopsy samples. IL-15 transcripts were detected in all renal biopsy specimens and were significantly increased in specimens obtained from rejecting as compared with nonrejecting renal allografts. IL-15 transcription correlates with rejection and may play an important role in T-cell mediated rejection.