IL-12 indirectly enhances proliferation of 5-FU-treated human hematopoietic peripheral blood CD34+ cells
✍ Scribed by Grafte, Stéphanie; Lévêque, Catherine; Paysant, Jérôme; Vasse, Marc; Soria, Claudine; Vannier, Jean-Pierre
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 53 KB
- Volume
- 58
- Category
- Article
- ISSN
- 0361-8609
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✦ Synopsis
Interleukin-12 (IL-12) or natural killer cell stimulatory factor (NKSF), has multiple effects on T lymphocytes and natural killer cells. In this study, the effect of IL-12 on human hematopoiesis was studied by analyzing the growth of CD34 + peripheral blood stem cells (PBSC), in steady state. In the presence of Epo, IL-12 alone or in combination with IL-3 or SCF had no effect on the formation of colonies from CD34 + cells. In culture with Epo, G-CSF, and IL-3, the effect of Flt3-ligand (FL) on CD34 + PBSC was investigated in the presence or absence of IL-12. No additional effect of IL-12 was observed when combined with FL. We evaluated 5-FU-treated human CD34 + PBSC proliferation in cultures with Epo, G-CSF, and IL-3, in the presence or absence of IL-12. No cytokine combination enhanced colony formation from 5-FU-treated CD34 + cells. However, in cultures of 5-FU-treated human CD34 + cells, the most efficient combination was IL-3 + Epo + G-CSF + Accessory cells (CD34 -). Furthermore, IL-12 enhanced this colony formation significantly. To investigate whether immature CD34 + cells were responsible for FL or SCF, 5-FU-treated human CD34 + cells were cultured with or without IL-12. Whereas no synergistic effect was observed in combination with IL-12, SCF alone significantly enhanced colony formation. However, the colony number was found to be smaller than with the potent combination of accessory cells in the presence of IL-12. These results indicate that accessory cells, lost in CD34 + cell purification, could be partly responsible for an IL-12 effect on immature human PBSC proliferation. Am.