✦ LIBER ✦

IgM to human cytomegalovirus: Comparison of two enzyme immunoassays and igm reactivity to viral polypeptides detected by immunoblotting

✍ Scribed by M. C. Re; MD M. P. Landini

Publisher: John Wiley and Sons
Year: 1989
Tongue: English
Weight: 480 KB
Volume: 3
Category: Article
ISSN: 0887-8013
DOI: 10.1002/jcla.1860030307

No coin nor oath required. For personal study only.

✦ Synopsis

IgM detection by enzyme-linked immunosorbent assay (ELISA) is the most used method of establishing a cytomegalovirus (CMV) infection during pregnancy. In this paper, we discuss the results obtained assaying 1,000 sera from pregnant women by two ELISA kits: a traditional indirect ELISA and a more recent IgM-capture ELISA. All the sera that gave a positive ELISA value with one or both kits were further tested by immunoblotting (IB) to establish which CMV polypeptides were detected by IgM antibodies. From the results obtained, IgM-capture ELISA seems less sensitive than indirect ELISA, but correlates better with serological evidence of active CMV infections as judged by typical IB profiles.

📜 SIMILAR VOLUMES

Comparison of two Cobas Core enzyme immu

Comparison of two Cobas Core enzyme immunoassays with other test systems for the detection of cytomegalovirus-specific IgG and IgM antibodies

✍ Dr. J. Steinmann; T. Weigel 📂 Article 📅 1994 🏛 John Wiley and Sons 🌐 English ⚖ 902 KB

## Abstract The new Cobas Core CMV IGM and IgG enzyme immunoassays (Roche Diagnostic Systems, Basle, Switzerland) were evaluated for their ability to detect cytomegalovirus (CMV)‐specific IgM and IgG antibodies. Therefore, both were compared with some other commercially available and already establ