IgG2 and other immunoglobulin classes on the cell surface of rat lymphoid cells

✍ Scribed by A. F. Williams

• Publisher: John Wiley and Sons
• Year: 1975
• Tongue: English
• Weight: 291 KB
• Volume: 5
• Category: Article
• ISSN: 0014-2980
• DOI: 10.1002/eji.1830051216

✦ Synopsis

Abstract

The binding to rat lymphoid cells of the F(ab′)~2~ fragments of purified rabbit antibodies specific for the Fc or Fd part of rat IgG~2~ was studied. Both reagents heavily labeled about 15 % and 6 % of cells from spleen and cervical lymph nodes respectively of rats kept in a conventional animal house. In contrast to this there were very few IgG~2~ positive cells in the thoracic duct lymph of any animals, or in the spleen of rats from an SPF animal house.

Results on the quantitative binding of rabbit antibodies against rat Fab, IgM and IgA to rat lymphoid cells are also reported. IgM positive cells in the spleen bound much more anti‐IgM relative to their binding of anti‐Fab antibody, than IgM positive cells from thoracic duct lymph or cervical lymph node.