We have recently shown that intact IgGs from the sera of healthy Wistar rats oxidize 3,3 0 -diaminobenzidine (DAB) in the presence and in the absence of H 2 O 2 similar to horseradish peroxidase (HRP). Here we demonstrate for the first time that the peroxidase and oxidoreductase activities of IgGs c
IgG antibodies with peroxidase-like activity from the sera of healthy Wistar rats
✍ Scribed by Erdenechimeg N. Ikhmyangan; Nataliya L. Vasilenko; Valentina N. Buneva; Georgy A. Nevinsky
- Book ID
- 116465742
- Publisher
- Elsevier Science
- Year
- 2005
- Tongue
- English
- Weight
- 161 KB
- Volume
- 579
- Category
- Article
- ISSN
- 0014-5793
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📜 SIMILAR VOLUMES
We present evidence showing that a small fraction of electrophoretically homogeneous IgGs from the sera of healthy Wistar rats is bound with several different Me 2þ ions and oxidizes 3,3 0 -diaminobenzidine through a peroxidase activity in the presence of H 2 O 2 and through an oxidoreductase activi
## Abstract It was shown that IgGs purified from the sera of healthy Wistar rats contain several different bound Me^2+^ ions and oxidize 3,3′‐diaminobenzidine through a H~2~O~2~‐dependent peroxidase and H~2~O~2~‐independent oxidoreductase activity. IgGs have lost these activities after removing the
MATERIAL AND METHODS ## Reagents PtdIns and BSA were purchased from Sigma (St Louis, MO). Freund's complete (Bacto H37 Ra) and incomplete adjuvants were purchased from DIFCO (Detroit, MI).