Targeted mutagenesis identifies Asp-569
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Rechinsky, Vladimir O. ;Chernov, Boris K. ;Dragan, Sofia M. ;Kostyuk, Dmitry A.
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Article
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1995
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Springer
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English
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In order to look more closely at a wellconserved region in T7 RNA polymerase (T7 RNAP) containing, as shown earlier, the functionally essential residues Pro-563 and Tyr-571, we used targeted mutagenesis to change those residues within this region that are invariant in all single-subunit RNA polymera