## Abstract Phosphorylation of proteins is an important postβtranslational protein modification in cellular response to environmental change and occurs in both prokaryotes and eukaryotes. Identification of the amino acid on individual proteins that become phosphorylated in response to extracellular
Identification of Tyrosine-Phosphorylated Peptides Using Cold Ion Spectroscopy
β Scribed by Kopysov, Vladimir; Nagornova, Natalia S.; Boyarkin, Oleg V.
- Book ID
- 127194012
- Publisher
- American Chemical Society
- Year
- 2014
- Tongue
- English
- Weight
- 618 KB
- Volume
- 136
- Category
- Article
- ISSN
- 0002-7863
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A simple procedure is described for determining the location of phosphorylation sites in phosphopeptides. The method employs measurement of 32P-labeled inorganic phosphate release during Edman degradation cycles using a gas-phase sequencer. The procedure is based on extracting peptides and inorganic