𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Identification of two cell surface proteins of rat brain oligodrocytes in culture

✍ Scribed by F. M. Pari; A. Espinosa de los Monteros; G. Roussel; Dr. J. L. Nussbaum

Publisher
John Wiley and Sons
Year
1987
Tongue
English
Weight
580 KB
Volume
17
Category
Article
ISSN
0360-4012

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✦ Synopsis

Antibodies specific for the surface of oligodendrocytes were prepared by incubating living cultures of pure oligodendrocytes with a crude anti-oligodendrocyte antiserum. These specific antibodies, when used in the technique of immunoelectroblotting, led to the characterization of at least two major plasma membrane proteins of 43 kilodaltons (kDa) and 53 kDa, respectively, as accessible at the external surface of the oligodendrocytes. The 53-kDa protein was also found in oligodendrocyte-conditioned medium in significant amounts. Additional oligodendrocyte surface proteins were also detected in the Wolfgram protein fraction.

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