Identification of triacylglycerol positional isomers present in vegetable oils by high performance liquid chromatography/atmospheric pressure chemical ionization mass spectrometry

✍ Scribed by Hazel R. Mottram; Simon E. Woodbury; Richard P. Evershed

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 302 KB
• Volume: 11
• Category: Article
• ISSN: 0951-4198
• DOI: 10.1002/(sici)1097-0231(199708)11:12<1240::aid-rcm990>3.0.co;2-5

✦ Synopsis

High performance liquid chromatography in conjunction with atmospheric pressure chemical ionization mass spectrometry (HPLC/APCI-MS) enables the positional isomers of individual triacylglycerols in mixtures to be identified. Application of HPLC/APCI-MS to the analysis of soybean oil allowed thirty nine triacylglycerols and seven diacylglycerols to be positively identified. The compounds present were quantified and the distribution of fatty acids in the β-position calculated. This distribution compared favourably with that obtained using a more conventional regiospecific lipase degradation of the oil. A survey of the acylglycerol components of a range of vegetable oils (blackcurrant, blue poppy seed, evening primrose, extra virgin olive, hazelnut, maize and rapeseed) carried out using HPLC/APCI-MS is reported.