Identification of tomato cultivars (Lycopersicon esculentum) by polyacrylamide isoelectric focusing

✍ Scribed by Gisela Henn; A. W. H. Neitz; A. I. Louw

• Book ID: 104617513
• Publisher: Springer
• Year: 1992
• Tongue: English
• Weight: 396 KB
• Volume: 62
• Category: Article
• ISSN: 0014-2336
• DOI: 10.1007/bf00037931

✦ Synopsis

Isozyme analyses have been used for the definitive identification of many plant cultivars, but not for cultivated tomatoes . Six isozyme systems, namely alcohol dehydrogenase, acid phosphatase, phosphoglucomutase, esterase, phosphoglucoisomerase and 6-phosphogluconate dehydrogenase of tomato seed extracts were resolved by isoelectric focusing on polyacrylamide gels with a narrow pH gradient . Nine alcohol dehydrogenase phenotypes were distinguished which, with three acid phosphatase phenotypes, identified twelve of the seventeen cultivars . Fewer differences were found for the other isozymes . Since this method could differentiate between breeding parents and their progeny it is concluded that further investigations are warranted .