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Identification of the transcribing DNA strand for the rII and endolysin genes of coliphage T4

โœ Scribed by Kasai, Takashi; Bautz, E.K.F.; Guha, A.; Szybalski, W.

Book ID
122416029
Publisher
Elsevier Science
Year
1968
Tongue
English
Weight
270 KB
Volume
34
Category
Article
ISSN
0022-2836

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Construction and properties of recombinant plasmids containing the rII genes of bacteriophage T4
โœ Selzer, Gerald ;Bolle, Antoinette ;Krisch, Henry ;Epstein, Richard ๐Ÿ“‚ Article ๐Ÿ“… 1978 ๐Ÿ› Springer ๐ŸŒ English โš– 920 KB

The EcoRI digestion products of phage T4 DNA have been examined using a phage DNA transformation assay. A 2.6 X 10(6) Dalton fragment was found to contain the rII genes. This fragment was purified and then treated with HindIII endonuclease. The cleavage products were ligated to the vector plasmid pB