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Identification of the minimal replication region of the multicopy Streptomyces plasmid pSL1

โœ Scribed by Yutaka Shindoh; Hiroaki Urabe; Michiko M. Nakano; Hiroshi Ogawara


Book ID
119078911
Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
592 KB
Volume
17
Category
Article
ISSN
0147-619X

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An amber replication mutant of F plasmid
โœ Maki, Satoko ;Kuribayashi, Minoru ;Miki, Takeyoshi ;Horiuchi, Tadao ๐Ÿ“‚ Article ๐Ÿ“… 1983 ๐Ÿ› Springer ๐ŸŒ English โš– 731 KB

We have constructed a mini-F derivative (pKP1013) consisting of a 5.4 kilobase pairs (kb) segment (44.0 to 49.4 kb) of mini-F and fragments carrying the chloramphenicol and spectinomycin resistance genes that originated from the R plasmid NR1. The plasmid pKP1013 replicates autonomously in a manner