Identification of the Luciferin–Luciferase System and Quantification of Coelenterazine by Mass Spectrometry in the Deep-Sea Luminous Ostracod Conchoecia pseudodiscophora

✍ Scribed by Yuichi Oba; Hiroshi Tsuduki; Shin-ichi Kato; Makoto Ojika; Satoshi Inouye

• Book ID: 101822020
• Publisher: John Wiley and Sons
• Year: 2004
• Tongue: English
• Weight: 212 KB
• Volume: 5
• Category: Article
• ISSN: 1439-4227
• DOI: 10.1002/cbic.200400102

✦ Synopsis

Abstract

The bioluminescence system of the ostracod Conchoecia pseudodiscophora__, which is abundant in the Sea of Japan, has been characterized. The luminescence (λ~max~=463 nm) is produced by a luciferin–luciferase reaction, and the luciferin has been identified as coelenterazine. Coelenterazine, coelenteramide, and coelenteramine from__ C. pseudodiscophora were quantified by LC‐ESI‐MS/MS analysis. The coelenterazine content was estimated to be approximately 230 pg per animal by using a calibration curve of synthetic coelenterazine. The reaction between homogenates of C. pseudodiscophora and synthetic coelenterazine showed luminescence activity; this suggests that a coelenterazine‐type luciferase is present.