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Identification of the genes in multicopy plasmids affecting ompC and ompF expression in Escherichia coli

✍ Scribed by Tian Jin; Masayori Inouye

Book ID
109320847
Publisher
John Wiley and Sons
Year
1995
Tongue
English
Weight
619 KB
Volume
133
Category
Article
ISSN
0378-1097

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The btuB gene of Escherichia coli codes for a protein (BtuB) located in the outer membrane. BtuB is the receptor for vitamin B12 (cyanocobalamin). We have cloned the btuB gene into pUC8 using transposon Tn5 as the marker to first isolate several parts of the relevant DNA fragment from the specialize