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Identification of the gal3 insertion in Escherichia coli AS IS2

✍ Scribed by Fiandt, M.; Szybalski, W.; Ahmed, A.


Book ID
122900295
Publisher
Elsevier Science
Year
1977
Tongue
English
Weight
366 KB
Volume
2
Category
Article
ISSN
0378-1119

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Transposition events mediated by plasmidborne copies of the insertion sequence IS3 of Escherichia coli are difficult to detect because of a low frequency of cointegrate formation. We found that cointegration activity could be strongly enhanced by using plasmid constructions in which a second IS3 ele