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Identification of the E2-binding residues in the N-terminal domain of E1 of a prokaryotic pyruvate dehydrogenase complex

✍ Scribed by Annechien F Hengeveld; Aart de Kok


Book ID
117104358
Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
153 KB
Volume
522
Category
Article
ISSN
0014-5793

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Most of the mutations causing deficiency of the pyruvate dehydrogenase (PDH) complex are in the X-linked E,a gene. We have developed a rapid screening method for the detection of mutations in this gene using reverse transcription of total RNA, polymerase chain reaction amplification of the whole cod