Matrix-assisted laser desorption/ionization time-of-flight mass spectra of proteins in cerebrospinal fluid analyzed without prior purification are presented. Less than 100 fmol amounts of proteins in the 10 000 to 20 000 u mass range and linked to human disease (multiple sclerosis, Alzheimer's disea
Identification of the apolipoprotein E4 isoform in cerebrospinal fluid with preparative two-dimensional electrophoresis and matrix assisted laser desorption/ionization-time of flight-mass spectrometry
β Scribed by Camilla Hesse; Carl L. Nilsson; Kaj Blennow; Pia Davidsson
- Publisher
- John Wiley and Sons
- Year
- 2001
- Tongue
- English
- Weight
- 69 KB
- Volume
- 22
- Category
- Article
- ISSN
- 0173-0835
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
A novel combination of methods, two-dimensional liquid-phase electrophoresis (2D-LPE) and matrixassisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS), have been used for the analysis of intact brain-specific proteins in cerebrospinal fluid (CSF). 2D-LPE is especially us
Matrix selection and sample preparation technique, namely finding a suitable matrix-analyte combination, has been one of the most important concerns in applying positive and negative ion matrix-assisted laser desorption/ionization (MALDI) mass spectrometry to synthetic polymers. Most matrices used i