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Identification of the 1H resonances of valine and leucine residues in dihydrofolate reductase by using a combination of selective deuteration and two-dimensional correlation spectroscopy

✍ Scribed by M.S. Searle; S.J. Hammond; B. Birdsall; G.C.K. Roberts; J. Feeney; R.W. King; D.V. Griffiths


Book ID
115917700
Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
572 KB
Volume
194
Category
Article
ISSN
0014-5793

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Assignment of resonances in the 1H spect
✍ Kevin L. Behar; Takashi Ogino πŸ“‚ Article πŸ“… 1991 πŸ› John Wiley and Sons 🌐 English βš– 1011 KB

Two-dimensional shift-correlated (COSY) and J-resolved NMR spectroscopy was used to identify and assign resonance in the aliphatic region (0.8 to 4.5 ppm) of the 1H spectrum of acid extracts and tissue of rat brain. The chemical shift and spin-spin coupling constants of several resonances, which cou