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Identification of single eukaryotic cells with micro-Raman spectroscopy

✍ Scribed by P. Rösch; M. Harz; K.-D. Peschke; O. Ronneberger; H. Burkhardt; J. Popp

Publisher
Wiley (John Wiley & Sons)
Year
2006
Tongue
English
Weight
198 KB
Volume
82
Category
Article
ISSN
0006-3525

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✦ Synopsis

Abstract

For a fast identification of eukaryotic cells such as yeast species without a cultivation step it should be possible to perform the investigation on only one single cell. Since yeasts as eukaryotes are heterogeneous and their Raman spectra are therefore dependent on the measuring position, one Raman spectra is not representative of the whole cell. In this contribution we demonstrate the application of average Raman spectra of a line scan over single yeast cells. These average spectra are used for classification with the help of a support vector machine. © 2006 Wiley Periodicals, Inc. Biopolymers 82:312–316, 2006

This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at [email protected]

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