✦ LIBER ✦

Identification of semen in stain by determination of the specific activity of l-tartrate-inhibitable acid phosphatase

✍ Scribed by Tamaki, Keiji ;Fujisawa, Kazue ;Okajima, Hiroshi ;Sato, Keizo ;Katsumata, Yoshinao

Publisher: Springer-Verlag
Year: 1989
Tongue: English
Weight: 460 KB
Volume: 102
Category: Article
ISSN: 1437-1596
DOI: 10.1007/bf00204073

No coin nor oath required. For personal study only.

✦ Synopsis

For identification of semen in stain the specific activity of Ltartrate-inhibitable acid phosphatase (ACP) was determined. With each stain extract, both enzyme activity and protein concentration were determined, and the specific activity (enzyme activity/protein concentration) was calculated. Seminal stains showed a value of 23.8 _+ 15.2 (mean _+ SD) IU/mg protein, while vaginal fluid stains showed a value of 0.088 _+ 0.049 IU/mg protein. Stains of other body fluids did not show any L-tartrate-inhibitable ACP activity. Furthermore, only eight of 30 plant juice stains showed any levels of L-tartrate-inhibitable ACP, although all plants tested showed ACP activity. As the present method enables us to analyze forensic samples quantitatively, it seems to be useful for forensic practice.

📜 SIMILAR VOLUMES

Identification of saliva stains by deter

Identification of saliva stains by determination of the specific activity of amylase

✍ Hajime Tsutsumi; Koji Higashide; Yasushi Mizuno; Keiji Tamaki; Yoshinao Katsumat 📂 Article 📅 1991 🏛 Elsevier Science 🌐 English ⚖ 469 KB

The specific activity (enzyme activity/protein concentration) of amylase was determined for the identification of saliva stains. The specific activity of amylase in saliva stains rapidly decreased during the first hour but, from 1 to 28 days, this decrease was much less when the stains were kept at

Inhibition of firing rate and changes in

Inhibition of firing rate and changes in the firing pattern of nigral dopamine neurons by γ-hydroxybutyric acid (GHBA) are specifically induced by activation of GABAB receptors

✍ S. Erhardt; Bengt Andersson; Hans Nissbrandt; Göran Engberg 📂 Article 📅 1998 🏛 Springer-Verlag 🌐 English ⚖ 228 KB

A rapid and simple method to determine t

A rapid and simple method to determine the specific activities of serotonin, 5-hydroxyindoleacetic acid, and 5-hydroxytryptophan in brain by HPLC with electrochemical detection

✍ M. J. Bernstein; P. A. Shea 📂 Article 📅 1982 🏛 Springer 🌐 English ⚖ 377 KB

Erratum to “multistep collisionally acti

Erratum to “multistep collisionally activated decomposition in an ion trap for the determination of the amino-acid sequence and gas-phase ion chemistry of lithium-coordinated valinomycin,” by Lambert C.M. Ngoka and Michael L. Gross (Int. J. Mass Spectrom. 194 (2000) 247–259)

📂 Article 📅 2000 🏛 Elsevier Science 🌐 English ⚖ 66 KB