✦ LIBER ✦
Identification of S-locus linked PCR fragments in rye (Secale cereale L.) by denaturing gradient gel electrophoresis
✍ Scribed by P. Wehling; B. Hackauf; G. Wricke
- Publisher
- John Wiley and Sons
- Year
- 1994
- Tongue
- English
- Weight
- 554 KB
- Volume
- 5
- Category
- Article
- ISSN
- 0960-7412
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✦ Synopsis
Summary
Rye inbred lines segregating at the S‐locus and homozygous at the Z‐locus were investigated by PCR with primers derived from Brassica SLG‐sequences. After denaturing gradient gel electrophoresis (DGGE), a 280 bp PCR‐fragment displays a polymorphism perfectly correlated to the underlying S‐genotypes. This is the first report on S‐related DNA polymorphism in a bifactorial self‐incompatibility system of the Poaceae.