✦ LIBER ✦

Identification of radiation-induced cross-linking between thymine and tryptophan by electrospray ionization-mass spectrometry

✍ Scribed by Wei-Qun Shi; Jia Hu; Wei Zhao; Xiao-Yang Su; Hui Cai; Yu-Fen Zhao; Yan-Mei Li

Publisher: John Wiley and Sons
Year: 2006
Tongue: English
Weight: 560 KB
Volume: 41
Category: Article
ISSN: 1076-5174
DOI: 10.1002/jms.1090

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✦ Synopsis

Abstract

Upon exposure to ionizing radiation, DNA undergoes a variety of modifications including the production of a covalent bond between the nucleobase thymine and aromatic amino acids. In this work, electrospray ionization‐mass spectrometry(ESI‐MS) was used to identify the gamma radiation‐induced covalent cross‐linking of model peptides (sequence YPPW and pYPPW) with the nucleobase thymine. Tandem electrospray ionization‐mass spectrometry (ESI‐MS^n^) was employed to investigate the cross‐linking sites. The results showed that irrespective of whether tyrosine was phosphorylated or not, the nucleobase thymine was cross‐linked with the tryptophan residue. Possible cross‐linking mechanisms are proposed by investigating the related mass peaks. Copyright © 2006 John Wiley & Sons, Ltd.

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