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Identification of proteins that interact with catalytically active calcium-dependent protein kinases fromArabidopsis

โœ Scribed by Yuichi Uno; Miguel A. Rodriguez Milla; Eileen Maher; John C. Cushman

Book ID
106132245
Publisher
Springer
Year
2009
Tongue
English
Weight
987 KB
Volume
281
Category
Article
ISSN
1617-4615

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โœ N.H. Battey; M.A. Venis ๐Ÿ“‚ Article ๐Ÿ“… 1988 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 1020 KB

To determine whether a Ca2+-activated protein kinase is regulated by calmodulin, it is necessary to separate it from endogenous calmodulin and from protein kinase activity that is not calcium dependent. We describe here a procedure for achieving these goals using Ca2+-dependent hydrophobic interacti