Identification of proteins by crossed immunoelectrophoresis with a trap-gel

✍ Scribed by A. Laine; M.P. Ducourouble; M.H. Hannothiaux

• Book ID: 102988707
• Publisher: Elsevier Science
• Year: 1987
• Tongue: English
• Weight: 740 KB
• Volume: 161
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(87)90648-8

✦ Synopsis

Simple improvements of the crossed immunoelectrophoresis method are described. A trapgel was prepared with a small quantity of monospecific antiserum and was submitted to preelectrophoresis. It blocked, during the first dimension, an individual protein as a rocket. The corresponding peak was reduced or disappeared in the second dimension. Identification of precipitation peaks in human or Macacus cynomolgus bronchoalveolar lavage fluids is illustrated and unequivocal recognition of some antigens is shown. 0 1987 Academic press, LX.