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Identification of Polysomes Synthesizing Thyroglobulin

โœ Scribed by Gilbert V Assart; Jacques E. DUMONT

Book ID
115113462
Publisher
John Wiley and Sons
Year
1973
Tongue
English
Weight
765 KB
Volume
32
Category
Article
ISSN
1432-1327

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๐Ÿ“œ SIMILAR VOLUMES

Improved techniques for the isolation of
Improved techniques for the isolation of intact thyroglobulin-synthesizing polysomes
โœ Eric Davies; J.E. Dumont; G. Vassart ๐Ÿ“‚ Article ๐Ÿ“… 1977 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 490 KB

A method is presented, based on the use of an extraction buffer containing 200 mM Tris-HCl, pH 8.5, which permits the isolation in high yield of apparently undegraded polysomes from beef thyroid. Upon treatment with detergent, both the 8OOg pellet and the 27,000g pellet yield polysome profiles exhib