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Identification of platelet proteins separated by two-dimensional gel electrophoresis and analyzed by matrix assisted laser desorption/ionization-time of flight-mass spectrometry and detection of tyrosine-phosphorylated proteins

✍ Scribed by Katrin Marcus; Dorian Immler; Joachim Sternberger; Helmut E. Meyer


Publisher
John Wiley and Sons
Year
2000
Tongue
English
Weight
971 KB
Volume
21
Category
Article
ISSN
0173-0835

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A nonradioactive assay for protein tyrosine phosphatases (PTPs), employing a tyrosine-phosphorylated peptide as a substrate, has been developed and applied to analyze purified enzymes, cell extracts, and immunoprecipitates. The reaction was followed by matrix-assisted laser desorption ionization tim