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Identification of pig chromosomes in pig-mouse somatic cell hybrid bivariate flow karyotypes

✍ Scribed by Alain Bouvet; Bernard A. Konfortov; Nigel G. A. Miller; David Brown; Dr. Elizabeth M. Tucker

Publisher
John Wiley and Sons
Year
1993
Tongue
English
Weight
727 KB
Volume
14
Category
Article
ISSN
0196-4763

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✦ Synopsis

To identify pig chromosomes in pigmouse somatic cell hybrids, dual-laser flow karyotypes and GTG-banded metaphase spreads of pig, mouse, and 7 p i g mouse hybrid cell lines were compared. Pig chromosomes no. 1,2,5,6,10,11,13, 14, 16, 18, X and Y were tentatively assigned to individual peaks in the pig flow karyotype on the basis of DNA content vs. relative chromosome length. In the 7 hybrid cell lines, 7 out of 8 peaks distinct from those of the mouse cell line could be correlated with the presence of pig chromosomes no. 5,9, 10, 11 or 16, 14, 15, and 18, whereas 1 peak appeared to correspond to the presence of 1 middle-size chromosome (3, 4, or 7). Other pig chromosomes present in the hybrids could not be detected with certainty due to superposition with mouse peaks and mouse chromosome rearrangements.

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