After oxidation of indole-3-acetic acid with ceric nitrate in the presence of hydroxylamine, a stable colored complex extractable to isoamyl alcohol is formed. This reaction is useful for the calorimetric estimation of indole-3-acetic acid in biological mixtures. The colored complex (whose chromopho
Identification of oxindole-3-acetic acid, and metabolic conversion of indole-3-acetic acid to oxindole-3-acetic acid inPinus sylvestrisseeds
✍ Scribed by Arild Ernstsen; Göran Sandberg; Karin Lundström
- Publisher
- Springer-Verlag
- Year
- 1987
- Tongue
- English
- Weight
- 516 KB
- Volume
- 172
- Category
- Article
- ISSN
- 0032-0935
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✦ Synopsis
Oxindole-3-acetic acid (OxIAA) has been identified in germinating seeds of Scots pine (Pinus sylvestris) using gas chromatography-mass spectrometry. Seeds germinated for 5 d contained 2.7 ng OxIAA·g(-1) (dry weight) whereas ungerminated seeds contained 0.2 ng·g(-1). Isotopically labelled OxIAA was formed in seeds incubated with [1'-(14)C]-, [2'-(14)C]- or [(2)H5]indole-3-acetic acid.
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