Identification of oleic acid binding sites in cytolysin A-III from the heteronemertine Cerebratulus lacteus

Identification of functioning regulatory

Identification of functioning regulatory sites and a new myosin binding site in the C-terminal 288 amino acids of caldesmon expressed from a human clone

✍ P. A. J. Huber; C. S. Redwood; N. D. Avent; M. J. A. Tanner; S. B. Marston 📂 Article 📅 1993 🏛 Springer Netherlands 🌐 English ⚖ 861 KB

A partial clone of caldesmon, coding for the C-terminal 288 amino acids, was isolated from a human fetal liver cDNA library and sequenced. Expression of the clone in Escherichia coli produced a peptide called H1 (M r 32 549), which inhibited tropomyosin-enhanced actomyosin Mg2+-ATPase activity by 90