Identification of neuropeptides in rat brain rhinencephalon

✍ Scribed by Albena Mihailova; Bartosz Karaszewski; Roman Hauser; Elsa Lundanes; Tyge Greibrokk

• Book ID: 102924573
• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 962 KB
• Volume: 30
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.200600317

✦ Synopsis

Abstract

A capillary two‐dimensional liquid chromatography method coupled with ion trap mass spectrometry has been used for separation and identification of neuropeptides in rat rhinencephalon. Animals of three different age groups were exposed to slow and quick CO~2~ influx. The neuropeptides were extracted by solid phase extraction and the purified extracts were analysed by 2‐D HPLC. The compounds were fractionated (strong cation exchange column), trapped and separated, and MS/MS fragment mass spectra were used for identification. About thirty peptide compounds were identified. A significant difference between concentration levels of “stressed” (quick CO~2~ influx) and “non‐stressed” (slow CO~2~ influx) rats was found for 25 of the identified peptides.