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Identification of Neighbouring Proteins in the Ribosomes of Escherichia coli : A Topographical Study with the Cross-Linking Reagent Dimethyl Suberimidate

โœ Scribed by Christopher Clegg; Donal Hayes


Book ID
115114011
Publisher
John Wiley and Sons
Year
1974
Tongue
English
Weight
655 KB
Volume
42
Category
Article
ISSN
1432-1327

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The use of sym-triazine trichloride in R
โœ Oste, Christian ;Brimacombe, Richard ๐Ÿ“‚ Article ๐Ÿ“… 1979 ๐Ÿ› Springer ๐ŸŒ English โš– 578 KB

The reagent sym-triazine trichloride is used as a bifunctional reagent to generate RNA-protein cross-links within intact ribosomal subunits from E. coli. The reaction takes place in a stepwise manner, involving substitution of one chlorine atom at 12 degrees and pH 8, and substitution of the second